Glycosylated hemoglobin A2 components

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Glycosylated hemoglobin A2 components.

Partially purified hemoglobin A2 has been examined for the existence of glycosylated components by isoelectric focusing and by acid agar gel electrophoresis. Bands analogous to the glycohemoglobin derivatives of hemoglobin A, hemoglobin-A1.a.b.c, were readily detected. Evidence that these minor bands are in fact glycohemoglobins was obtained by showing that 14C-glucose bound to hemoglobin A2 mo...

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Glycosylated Minor Components of Human Adult Hemoglobin

Human hemolysate contains several minor components designated Hb A,,, Hb Alh, Hb A,,, which are post-translational modifications of the major hemoglobin component A,,. Individuals with diabetes mellitus have elevated levels of Hb A,,, a hemoglobin modified with a glucose moiety at the NH, terminus of each /3 chain. A new chromatographic technique using Bio-Rex 70 is described which not only all...

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Glycosylated Hemoglobin in Perspective

With each new tool the insurance industry obtains to evaluate both states of health and states of disease, the temptation exists to use it in as many contexts as possible. The initial understanding in the mid-1960’s that diabetic individuals have different percentages of specific hemoglobins has led to the study and refinement of the hemoglobin Alc (HbAlc), which is being used increasingly by c...

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Functional properties of the glycosylated minor components of human adult hemoglobin.

The functional properties of four glycosylated minor components of human hemolysate have been examined. In 0.1 M potassium phosphate buffer, pH 7 and 2O”C, the p50 values of Hb Al,,, Hb Alaz, Hb Al,, and Hb A, were 14,9.8,6.3, and 7.4 mm Hg, respectively, compared to 7.8 mm Hg for the major component, Hb Ao. The A log p&A pH (pH 7 to pH 8) was -0.5 for Hb A1 .1, Hb Alaz, and Hb A1, as compared ...

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Improved colorimetric assay for glycosylated hemoglobin.

This colorimetric assay for glycosylated hemoglobin can be performed in 120 min with equipment available in most clinical laboratories. The glucose moiety of glycosylated hemoglobin is converted to 5-hydroxymethylfurfural by heating with oxalic acid for 60 min is an autoclave at 124 degrees C and 124 kPa (18 lb/in.2). The adduct formed by reacting 2-thiobarbituric acid with hydroxymethylfurfura...

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ژورنال

عنوان ژورنال: Blood

سال: 1980

ISSN: 0006-4971,1528-0020

DOI: 10.1182/blood.v56.3.571.571